Flt-1 (phospho Tyr1333) Polyclonal Antibody

Rabbit Anti-Flt-1 (phospho Tyr1333) Polyclonal Antibody

Product Specifications

Background

FLT1 encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig) -like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. fms related tyrosine kinase 1 binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for FLT1.Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.

CAS Number

9007-83-4

Product Name Alternative

FLT1; FLT; FRT; VEGFR1; Vascular endothelial growth factor receptor 1; VEGFR-1; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; FLT; Vascular permeability factor receptor

Gene ID

2321

Accession Number

P17948

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Flt-1 around the phosphorylation site of Y1333

Clonality

Polyclonal

Tag

Vascular endothelial growth factor receptor 1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

151kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Flt-1 (Y1333) Polyclonal Antibody detects endogenous levels of Flt-1 protein only when phosphorylated at Y1333.

Observed Molecular Weight

151

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:100-300; Not yet tested in other applications.