Syk (phospho Tyr525) Polyclonal Antibody

Rabbit Anti-Syk (phospho Tyr525) Polyclonal Antibody

Product Specifications

Background

SYK encodes a member of the family of non-receptor type Tyr protein kinases.Spleen associated tyrosine kinase is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for SYK.

CAS Number

9007-83-4

Product Name Alternative

SYK; Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk

Gene ID

6850

Accession Number

P43405

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthesized peptide derived from human Syk around the phosphorylation site of Y525

Clonality

Polyclonal

Tag

Tyrosine-protein kinase SYK

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

72kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Syk (Y525) Polyclonal Antibody detects endogenous levels of Syk protein only when phosphorylated at Y525.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200