Synaptotagmin 1/2 (phospho Ser309/306) Polyclonal Antibody

Rabbit Anti-Synaptotagmin 1/2 (phospho Ser309/306) Polyclonal Antibody

Product Specifications

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]) .

CAS Number

9007-83-4

Product Name Alternative

SYT1; SVP65; SYT; Synaptotagmin-1; Synaptotagmin I; SytI; p65; SYT2; Synaptotagmin-2; Synaptotagmin II; SytII

Gene ID

6857/127833

Accession Number

P21579/Q8N9I0

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Synaptotagmin 1/2 around the phosphorylation site of S309/306

Clonality

Polyclonal

Tag

Synaptotagmin-1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

47kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Synaptotagmin 1/2 (S309/306) Polyclonal Antibody detects endogenous levels of Synaptotagmin 1/2 protein only when phosphorylated at S309/306.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200