IRS-1 (phospho Ser307) Polyclonal Antibody

Rabbit Anti-IRS-1 (phospho Ser307) Polyclonal Antibody

Product Specifications

Background

IRS1 encodes a protein (insulin receptor substrate 1) which is phosphorylated by insulin receptor tyrosine kinase. Mutations in IRS1 are associated with type II diabetes and susceptibility to insulin resistance.

CAS Number

9007-83-4

Product Name Alternative

IRS1; Insulin receptor substrate 1; IRS-1

Gene ID

3667

Accession Number

P35568

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthesized peptide derived from human IRS-1 around the phosphorylation site of S307

Clonality

Polyclonal

Tag

Insulin receptor substrate 1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

170kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-IRS-1 (S307) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at S307.

Observed Molecular Weight

170

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200