Dok-2 (phospho Tyr299) Polyclonal Antibody

Rabbit Anti-Dok-2 (phospho Tyr299) Polyclonal Antibody

Product Specifications

Background

Docking protein 2 encoded by DOK2 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210 (bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 2 binds p120 (RasGAP) from CML cells.

CAS Number

9007-83-4

Product Name Alternative

DOK2; Docking protein 2; Downstream of tyrosine kinase 2; p56 (dok-2)

Gene ID

9046

Accession Number

O60496

Reactivity

Human, Mouse, Monkey

Immunogen

Synthesized peptide derived from human Dok-2 around the phosphorylation site of Y299

Clonality

Polyclonal

Tag

Docking protein 2

Applications

WB, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

48kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Dok-2 (Y299) Polyclonal Antibody detects endogenous levels of Dok-2 protein only when phosphorylated at Y299.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.