C-Kit (phospho Tyr721) Polyclonal Antibody

Rabbit Anti-c-Kit (phospho Tyr721) Polyclonal Antibody

Product Specifications

Background

KIT encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT proto-oncogene receptor tyrosine kinase is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor) . Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for KIT.

CAS Number

9007-83-4

Product Name Alternative

KIT; SCFR; Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD antigen CD117

Gene ID

3815

Accession Number

P10721

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human c-Kit around the phosphorylation site of Y721

Clonality

Polyclonal

Tag

Mast

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

110kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-c-Kit (Y721) Polyclonal Antibody detects endogenous levels of c-Kit protein only when phosphorylated at Y721.

Observed Molecular Weight

110

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.