Bcl-2 (phospho Thr56) Polyclonal Antibody

Rabbit Anti-Bcl-2 (phospho Thr56) Polyclonal Antibody

Product Specifications

Background

BCL2 encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

BCL2; Apoptosis regulator Bcl-2

Gene ID

596

Accession Number

P10415

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from human Bcl-2 around the phosphorylation site of T56

Clonality

Polyclonal

Tag

Apoptosis regulator Bcl-2

Applications

WB, IHC, IP, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

26kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Bcl-2 (T56) Polyclonal Antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at T56.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IP 2-5 ug:mg lysate; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.