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Histone H1 (Mono Methyl Lys25) Polyclonal Antibody

Rabbit Anti-Histone H1 (Mono Methyl Lys25) Polyclonal Antibody

Product Specifications

Background

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. HIST1H1A is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from HIST1H1A lack polyA tails but instead contain a palindromic termination element. HIST1H1A is found in the large histone gene cluster on chromosome 6.

CAS Number

9007-83-4

Product Name Alternative

HIST1H1A; H1F1; Histone H1.1; Histone H1a; HIST1H1C; H1F2; Histone H1.2; Histone H1c; Histone H1d; Histone H1s-1; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4

Gene ID

3024/3006/3007/3008

Accession Number

Q02539/P16403/P16402/P10412

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic Peptide

Clonality

Polyclonal

Tag

Histone H1.1

Applications

WB

Purification Method

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Molecular Weight

17-25kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous Histone H1 (Mono Methyl Lys25) protein.

Observed Molecular Weight

17-25

Formulation

Liquid solution

Buffer pH

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1000

Other Product Names

HIST1H1A; H1F1; Histone H1.1; Histone H1a; HIST1H1C; H1F2; Histone H1.2; Histone H1c; Histone H1d; Histone H1s-1; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Mouse

Accession Number URL

http://www.uniprot.org/uniprot/Q02539

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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