Nrf2 (Acetyl Lys599) Polyclonal Antibody

Rabbit Anti-Nrf2 (Acetyl Lys599) Polyclonal Antibody

Product Specifications

Background

NFE2L2 encodes a transcription factor (nuclear factor, erythroid 2 like 2) which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for NFE2L2.

CAS Number

9007-83-4

Product Name Alternative

NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor, erythroid derived 2, like 2

Gene ID

4780

Accession Number

Q16236

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the C-terminal region of human Nrf2 around the acetylation site of K599

Clonality

Polyclonal

Tag

Nuclear factor erythroid 2-related factor 2

Applications

WB, IHC, IF, ELISA

Field of Research

Cardiovascular Biology #Cell Biology #Epigenetics#Metabonomics

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

75-100kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Acetyl-Nrf2 (K599) Polyclonal Antibody detects endogenous levels of Nrf2 protein only when non-acetylation at K599.

Observed Molecular Weight

75-100

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:10000; IF 1:50-200

Other Product Names

NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor, erythroid derived 2, like 2