HMG-1 (Acetyl Lys12) Polyclonal Antibody

Rabbit Anti-HMG-1 (Acetyl Lys12) Polyclonal Antibody

Product Specifications

Background

HMGB1 encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. High mobility group box 1 plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of HMGB1 have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

CAS Number

9007-83-4

Product Name Alternative

HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1

Gene ID

3146

Accession Number

P09429

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the N-terminal region of human HMG-1 around the acetylation site of K12

Clonality

Polyclonal

Tag

High mobility group protein B1

Applications

WB, IHC, IF, ELISA

Field of Research

Epigenetics

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

30kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Acetyl-HMG-1 (K12) Polyclonal Antibody detects endogenous levels of HMG-1 protein only when non-acetylation at K12.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:10000; IF 1:50-200