NF-E4 (Acetyl Lys43) Polyclonal Antibody

Rabbit Anti-NF-E4 (Acetyl Lys43) Polyclonal Antibody

Product Specifications

Background

The erythroid-specific protein encoded by NFE4, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. NFE4 is located in an intron of the FBXL13 gene on the opposite strand.

CAS Number

9007-83-4

Product Name Alternative

NFE4; Transcription factor NF-E4

Gene ID

58160

Accession Number

Q86UQ8

Reactivity

Human

Immunogen

Synthesized peptide derived from the human NF-E4 around the acetylation site of K43

Clonality

Polyclonal

Tag

Transcription factor NF-E4

Applications

WB, ELISA, IHC

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

19kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Acetyl-NF-E4 (K43) Polyclonal AntibodySynthesized peptide derived from the human NF-E4 around the acetylation site of K43.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000