P53 (Di-Methyl-Lys370) Polyclonal Antibody

Rabbit Anti-p53 (Di-Methyl-Lys370) Polyclonal Antibody

Product Specifications

Background

TP53 encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains.Tumor protein p53 responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in TP53 are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of TP53 and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277) .

CAS Number

9007-83-4

Product Name Alternative

TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

Gene ID

7157

Accession Number

P04637

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from human p53 around the di-methylation site of K370

Clonality

Polyclonal

Tag

Cellular tumor antigen p53

Applications

IF, WB, IHC, ELISA

Field of Research

Cancer#Cell Biology #Epigenetics

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

53kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Di-Methyl-p53 (K370) Polyclonal Antibody detects endogenous levels of p53 protein only when di-methylated at K370.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200; WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; Not yet tested in other applications