His-tag Polyclonal Antibody
Rabbit Anti-His-tag Polyclonal Antibody
Product Specifications
Background
The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
CAS Number
9007-83-4
Product Name Alternative
His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag
Reactivity
Species independent
Immunogen
6X His synthetic peptide conjugated to KLH
Clonality
Polyclonal
Tag
His
Applications
WB, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
15kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
Observed Molecular Weight
15
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-1:3000; ELISA 1:20000; Not yet tested in other applications.
Other Product Names
His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Isotype
Rabbit IgG
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
127559-APC 100 µL
GRM6 (GRM6, GPRC1F, MGLUR6, Metabotropic Glutamate Receptor 6) (APC)
Sign In for Pricing
View Details