Cleaved-Thrombin APII (R327) Polyclonal Antibody

Rabbit Anti-Cleaved-Thrombin APII (R327) Polyclonal Antibody

Product Specifications

Background

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

F2; Prothrombin; Coagulation factor II

Gene ID

2147

Accession Number

P00734

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human Thrombin APII at AA range: 250-330

Clonality

Polyclonal

Tag

Prothrombin

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

19kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Cleaved-Thrombin APII (R327) Polyclonal Antibody detects endogenous levels of fragment of activated Thrombin APII protein resulting from cleavage adjacent to R327.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:20000; Not yet tested in other applications.