Cleaved-MMP-12 (G106) Polyclonal Antibody

Rabbit Anti-Cleaved-MMP-12 (G106) Polyclonal Antibody

Product Specifications

Background

MMP12 encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs) . Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. Matrix metallopeptidase 12 degrades soluble and insoluble elastin. MMP12 may play a role in aneurysm formation and mutations MMP12 are associated with lung function and chronic obstructive pulmonary disease (COPD) . MMP12 is part of a cluster of MMP genes on chromosome 11.

CAS Number

9007-83-4

Product Name Alternative

MMP12; HME; Macrophage metalloelastase; MME; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12

Gene ID

4321

Accession Number

P39900

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human MMP-12 at AA range: 60-140

Clonality

Polyclonal

Tag

Macrophage metalloelastase

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

42kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Cleaved-MMP-12 (G106) Polyclonal Antibody detects endogenous levels of fragment of activated MMP-12 protein resulting from cleavage adjacent to G106.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:20000; Not yet tested in other applications.