Collagen II Mouse Monoclonal Antibody (7F9)

Mouse Anti-Collagen II Mouse Monoclonal Antibody (7F9)

Product Specifications

Background

COL2A1 (collagen type II alpha 1 chain) encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in COL2A1 are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for COL2A1.

CAS Number

9007-83-4

Product Name Alternative

Collagen alpha-1 (II) chain; Alpha-1 type II collagen

Gene ID

1280

Accession Number

P02458

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic Peptide of Collagen II

Clonality

Monoclonal

Tag

Collagen alpha-1 (II) chain (Alpha-1 type II collagen) [Cleaved into: Collagen alpha-1 (II) chain; Chondrocalcin]

Applications

IHC, IF

Purification Method

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

142kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous Collagen II protein .

Observed Molecular Weight

142

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:50-300; IF 1:50-200