Acetyl NF kB P65 (K314/K315) Mouse Monoclonal Antibody (5G11)

Mouse Anti-Acetyl NF kB P65 (K314/K315) Mouse Monoclonal Antibody (5G11)

Product Specifications

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA (RELA proto-oncogene, NF-kB subunit), or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of RELA, RELA. Four transcript variants encoding different isoforms have been found for RELA.

CAS Number

9007-83-4

Product Name Alternative

Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Gene ID

5970

Accession Number

Q04206

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic Peptide of Acetyl NF kB P65 (K314/K315)

Clonality

Monoclonal

Tag

Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) (Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)

Applications

IHC, IF

Purification Method

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

65kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous Acetyl NF kB P65 (K314/K315) protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:50-300; IF 1:50-200