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HP-1α Mouse Monoclonal Antibody (5E3)

Mouse Anti-HP-1α Mouse Monoclonal Antibody (5E3)

Product Specifications

Background

CBX5 (chromobox 5) encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. CBX5 has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product Name Alternative

Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha

Gene ID

23468

Accession Number

P45973

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Protein of HP-1α

Clonality

Monoclonal

Tag

Chromobox protein homolog 5 (Antigen p25) (Heterochromatin protein 1 homolog alpha) (HP1 alpha)

Applications

IF, WB, IHC

Purification Method

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

22kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous HP-1α protein.

Product Datasheet

https://www.abbkine.com/datasheet/ABM40359.pdf

Observed Molecular Weight

22

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200; WB 1:500-2000; IHC 1:50-300

Other Product Names

Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Mouse

Accession Number URL

http://www.uniprot.org/uniprot/P45973

CAS Number

9007-83-4

Isotype

Mouse IgG1

Available Sizes

Curated Selection

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