Luciferase Mouse Monoclonal Antibody (2G11)
Mouse Anti-Luciferase Mouse Monoclonal Antibody (2G11)
Product Specifications
Background
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
CAS Number
9007-83-4
Accession Number
P08659
Reactivity
Firefly
Immunogen
Synthetic Peptide of Luciferase
Clonality
Monoclonal
Tag
Luciferase
Applications
WB
Purification Method
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml
Molecular Weight
60kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
Luciferase protein detects endogenous levels of Luciferase.
Observed Molecular Weight
60
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-2000
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Mouse
Accession Number URL
http://www.uniprot.org/uniprot/P08659
Isotype
Mouse IgG1
Available Sizes
Frequently Asked Questions
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