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Luciferase Mouse Monoclonal Antibody (6B8)

Mouse Anti-Luciferase Mouse Monoclonal Antibody (6B8)

Product Specifications

Background

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

CAS Number

9007-83-4

Accession Number

P08659

Reactivity

Firefly

Immunogen

Recombinant Protein of Luciferase of Luciferase

Clonality

Monoclonal

Tag

Luciferase

Applications

IF, WB

Purification Method

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Molecular Weight

60kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Luciferase protein detects endogenous levels of Luciferase.

Observed Molecular Weight

60

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200; WB 1:1000-2000

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Mouse

Accession Number URL

http://www.uniprot.org/uniprot/P08659

Isotype

Mouse IgG1

Available Sizes

Frequently Asked Questions

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