Anti-GABARAP Antibody

Rabbit Polyclonal Antibody specific to GABARAP

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GABA(A receptor-associated protein, MM46

Gene Name

GABARAP

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=GABARAP">GABARAP</a>

UniProt

O95166

Cellular Locus

Cytoplasmic vesicle, autophagosome membrane, Endomembrane system, Cytoplasm, cytoskeleton, Golgi apparatus membrane, Cytoplasmic vesicle

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to the C-terminus of human GABARAP

Target Antigen

γ-aminobutyric acid receptor-associated protein

Target

GABARAP

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed:9892355). Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538). Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205). Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205). Involved in apoptosis (PubMed:15977068). {PubMed:15169837, PubMed:15977068, PubMed:20562859, PubMed:22948227, PubMed:25684205, PubMed:31006538, PubMed:9892355}.

Additionnal Information

Immunoblotting: use at dilution of 1:1,000. A band of ~14-16kDa is detected. Other bands observed at ~40kDa (complex) and ~26kDa (dimer). Immunofluorescence: use at dilution of 1:100 These are recommended working dilutions. Endusers should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human and mouse GABARAP.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Gamma-aminobutyric acid A receptors (GABA(A) receptors) are ligand-gated chloride channels that mediate inhibitory neurotransmission. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. In addition, GABARAP has an important role in autophagy; it is crucial for autophagosome formation and sequestering cytosolic cargo into double-membrane vesicles which is then followed by degradation after fusion with lysosomes.