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Histone H3 Monoclonal Antibody

Mouse Anti-Histone H3 Monoclonal Antibody

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST1H3A is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from HIST1H3A lack polyA tails; instead, they contain a palindromic termination element. HIST1H3A is found in the large histone gene cluster on chromosome 6p22-p21.3.

CAS Number

9007-83-4

Product Name Alternative

HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/

Gene ID

8350/8351/8352/8353/8354/8355/8356/8357/8358/8968

Accession Number

P68431/Q71DI3/P84243

Reactivity

Zebrafish

Immunogen

Synthetic Peptide

Clonality

Monoclonal

Tag

Histone H3.1

Applications

WB

Field of Research

Epigenetics

Purification Method

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Molecular Weight

15kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects Zebrafish endogenous Histone H3 protein.

Observed Molecular Weight

15

Formulation

Liquid solution

Buffer pH

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-3000

Other Product Names

HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Mouse

Accession Number URL

http://www.uniprot.org/uniprot/P68431

Isotype

Mouse IgG1

Available Sizes

Curated Selection

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