LN28B rabbit pAb

The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012],

Product Specifications

Background

UniProt

Q6ZN17

Swiss Prot

Q6ZN17

Reactivity

Human; Mouse

Immunogen

Synthesized peptide derived from human protein . at AA range: 60-140

Target

LN28B

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

WB 1:500-2000 ELISA 1:5000-20000

Buffer

-20°C/1 year

Molecular Weight

27kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

27kD

Fragment

IgG

Subcellular Location

Nucleus. Nucleus, nucleolus . Cytoplasm . Predominantly nucleolar (PubMed:22118463) . In Huh7 cells, predominantly cytoplasmic, with only a subset of cells exhibiting strong nuclear staining; however, the specificity of the polyclonal antibody used in these experiments has not been not documented (PubMed:16971064) . .