MRCKG rabbit pAb

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. enzyme regulation: Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation. function: May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation. similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. similarity: Contains 1 AGC-kinase C-terminal domain. similarity: Contains 1 CNH domain. similarity: Contains 1 CRIB domain. similarity: Contains 1 PH domain. similarity: Contains 1 phorbol-ester/DAG-type zinc finger. similarity: Contains 1 protein kinase domain. subcellular location: Concentrates at the leading edge of cells. subunit: Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42. tissue specificity: Expressed in heart and skeletal muscle.