CENPF rabbit pAb

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

P49454

Swiss Prot

P49454

Reactivity

Human; Rat; Mouse

Immunogen

Synthesized peptide derived from human protein . at AA range: 2190-2270

Clonality

Polyclonal

Source

Rabbit

Applications

IHC; IF

Concentration

1 mg/ml

Dilution

IHC-p 1:50-300

Molecular Weight

353kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

353kD

Fragment

IgG

Subcellular Location

Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.