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Recombinant beta actin Monoclonal Antibody

Product Specifications

Background

Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR.

Abbreviation

Beta actin

UniProt

P60709; P60710; P60711

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Synthetic peptide within N-terminal residues of β-Actin

Target

ACTB; Actin beta; Actx; Actin cytoplasmic 1; b actin; A26C1A

Clonality

Recombinant

Clone

SA30-01

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Signal Transduction; Tags and Cell Markers; Isotype/Loading; Controls; Neuroscience; Biochemicals; Cancer; Cardiovascular; Cell Biology; Immunology; Metabolism; Epigenetics and Nuclear Signaling; Stem Cells

Purification

Protein A/G Purification

Concentration

1 mg/mL

Dilution

WB 1:5000-1:2000; IHC 1:500-1:1000; IF 1:500-1:1000

Buffer

PBS with 0.05% sodium azide, 0.1% protective protein and 40% glycerol, pH 7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Calculated Molecular Weight

43 kDa

Observed Molecular Weight

43 kDa

Isotype

IgG

Available Sizes

Frequently Asked Questions

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