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GRP78 BiP rabbit pAb

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell. [provided by RefSeq, Sep 2010],

Product Specifications

Background

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

Synonyms

78 kDa glucose-regulated protein (GRP-78) (Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78) (Heat shock 70 kDa protein 5) (Immunoglobulin heavy chain-binding protein) (BiP)

Gene ID

3309

UniProt

P11021

Cellular Locus

Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Cell surface. Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). Localizes to the cell surface of epithelial cells in response to high levels of free iron (PubMed:20484814, PubMed:24355926, PubMed:27159390)..

Host

Rabbit

Species Reactivity

Human,Mouse,Rat,Fish

Reactivity

Human; Mouse; Rat; Fish

Immunogen

Synthetic peptide from human protein at AA range: 505-570

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

WB, IHC, IF, ELISA

Validated Applications

WB,IHC,IF,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Western Blot: 1/500 - 1/2000.IHC-p/f:1:50-300. ELISA 1:10000-20000

Molecular Weight

78kD

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES8607-1.pdf

Observed Molecular Weight

78 kD

Subcellular Location

Endoplasmic reticulum lumen . Melanosome . Cytoplasm . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545) . Localizes to the cell surface of epithelial cells in response to high levels of free iron (PubMed:20484814, PubMed:24355926, PubMed:27159390) . .

Other Product Names

78 kDa glucose-regulated protein (GRP-78) (Endoplasmic reticulum lumenal Ca (2+) -binding protein grp78) (Heat shock 70 kDa protein 5) (Immunoglobulin heavy chain-binding protein) (BiP)

Gene ID (Human)

3309

SwissProt (Human)

P11021

Available Sizes

Curated Selection

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