P300 (Acetyl Lys1558/Acetyl Lys1560) rabbit pAb

E1A binding protein p300 (EP300) Homo sapiens This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008],

Product Specifications

Background

Product Name Alternative

EP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300

UniProt

Q09472

Swiss Prot

Q09472

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized acetyl-peptide derived from human p300 around the acetylation site of K1558.

Target

P300

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

300kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

300kD

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Chromosome . Localizes to active chromatin: Colocalizes with histone H3 acetylated and/or crotonylated at 'Lys-18' (H3K18ac and H3K18cr, respectively) (PubMed:25818647) . In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus (PubMed:12929931) . .