RNase III Drosha rabbit pAb

Drosha ribonuclease III (DROSHA) Homo sapiens This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016],

Product Specifications

Background

Product Name Alternative

DROSHA; RN3; RNASE3L; RNASEN; Ribonuclease 3; Protein Drosha; Ribonuclease III; RNase III; p241

UniProt

Q9NRR4

Swiss Prot

Q9NRR4

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human RNase III Drosha. AA range:774-823

Target

RNase III Drosha

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

160kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

160kD

Fragment

IgG

Subcellular Location

Nucleus . Nucleus, nucleolus . A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies. .