Centriolin rabbit pAb

Function: Necessary for centrosome duplication. Collaborates with CEP97, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2. induction: Up-regulated during the transition from G1 to S phase of the cell cycle. The highest levels are observed in S phase, after which the levels decrease markedly. PTM: Phosphorylated by CDKs. subunit: Interacts with CALM1, CETN2 and CEP97. tissue specificity: Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.

Product Specifications

Background

Function:Necessary for centrosome duplication. Collaborates with CEP97, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2., induction:Up-regulated during the transition from G1 to S phase of the cell cycle. The highest levels are observed in S phase, after which the levels decrease markedly., PTM:Phosphorylated by CDKs., subunit:Interacts with CALM1, CETN2 and CEP97., tissue specificity:Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.

UniProt

O43303

Swiss Prot

O43303

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human CEP110. AA range:850-900

Clonality

Polyclonal

Source

Rabbit

Applications

IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Storage Conditions

-20°C/1 year

Fragment

IgG

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, cilium basal body . Recruited early and then associates with the growing distal tips. Recruited to the mother centriole by KIF24 (PubMed:21620453) . Removed from centrioles by TTBK2, leading to initiation of ciliogenesis and localizes only to the daughter centriole in ciliated cells. In cytotoxic T lymphocytes remains associated with the mother centriole during docking of the centrosome at the immunological synapse upon target contact (By similarity) . Recruited at the distal end of the mother centriole by MPHOSPH9 (PubMed:30375385) . .