MAPKAPK-2 (phospho Thr222) rabbit pAb

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

P49137

Swiss Prot

P49137

Reactivity

Human; Mouse; Rat; Monkey

Immunogen

The antiserum was produced against synthesized peptide derived from human MAPKAPK-2 around the phosphorylation site of Thr222. AA range:188-237

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

45kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

45kD

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Phosphorylation and subsequent activation releases the autoinhibitory helix, resulting in the export from the nucleus into the cytoplasm.