Lfc (phospho Ser886) rabbit pAb

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2009],

Product Specifications

Background

UniProt

Q92974

Swiss Prot

Q92974

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Rho/Rac Guanine Nucleotide Exchange Factor 2 around the phosphorylation site of Ser885. AA range:851-900

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

111kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

111kD

Fragment

IgG

Subcellular Location

Cytoplasm, cytoskeleton . Cytoplasm . Cell junction, tight junction . Golgi apparatus . Cytoplasm, cytoskeleton, spindle . Cell projection, ruffle membrane . Cytoplasmic vesicle . Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization (PubMed:15827085) . Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells (PubMed:19043560) . Colocalized with NOD2 and RIPK2 in vesicles and with the cytoskeleton (PubMed:21887730) . .

Other Product Names

ARHGEF2; KIAA0651; LFP40; Rho guanine nucleotide exchange factor 2; Guanine nucleotide exchange factor H1; GEF-H1; Microtubule-regulated Rho-GEF; Proliferating cell nucleolar antigen p40