GANP rabbit pAb

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability. [provided by RefSeq, Jan 2014],

Product Specifications

Background

UniProt

O60318

Swiss Prot

O60318

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human GANP. AA range:1841-1890

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Molecular Weight

218kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

218kD

Fragment

IgG

Subcellular Location

[Isoform GANP]: Nucleus envelope . Nucleus, nuclear pore complex . Nucleus, nucleoplasm . Chromosome . Predominantly located at the nuclear envelope, facing the nucleus interior (PubMed:20005110, PubMed:21195085, PubMed:23591820) . Localization at the nuclear pore complex requires NUP153, TPR and ALYREF/ALY (PubMed:23591820, PubMed:22307388) . Also found associated with chromatin (PubMed:23652018) . In B-cells, targeted to the immunoglobulin variable region genes (PubMed:23652018) . .; [Isoform MCM3AP]: Cytoplasm . Nucleus . Translocates into the nucleus in the presence of MCM3 (PubMed:12226073) . Associates with chromatin possibly through interaction with MCM3 (PubMed:12226073) . .