Cdc16 (phospho Ser560) rabbit pAb

The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified. [provided by RefSeq, Jan 2016],

Product Specifications

Background

UniProt

Q13042

Swiss Prot

Q13042

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human CDC16/APC6 around the phosphorylation site of Ser560. AA range:526-575

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

72kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

72kD

Fragment

IgG

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.