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PIXβ rabbit pAb

This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016],

Product Specifications

Background

This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016]

Synonyms

ARHGEF7; COOL1; KIAA0142; P85SPR; PAK3BP; PIXB; Nbla10314; Rho guanine nucleotide exchange factor 7; Beta-Pix; COOL-1; PAK-interacting exchange factor beta; p85

Gene ID

8874

UniProt

Q14155

Cellular Locus

Cell junction, focal adhesion. Cell projection, ruffle. Cytoplasm, cell cortex. Cell projection, lamellipodium. Detected at cell adhesions. A small proportion is detected at focal adhesions.

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human ARHGEF7. AA range:311-360

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

WB, IHC, IF, ELISA

Validated Applications

WB,IHC,IF,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

80kD

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES7898-1.pdf

Observed Molecular Weight

80 kD

Subcellular Location

Cell junction, focal adhesion . Cell projection, ruffle . Cytoplasm, cell cortex . Cell projection, lamellipodium . Detected at cell adhesions. A small proportion is detected at focal adhesions.

Other Product Names

ARHGEF7; COOL1; KIAA0142; P85SPR; PAK3BP; PIXB; Nbla10314; Rho guanine nucleotide exchange factor 7; Beta-Pix; COOL-1; PAK-interacting exchange factor beta; p85

Gene ID (Human)

8874

SwissProt (Human)

Q14155

Available Sizes

Curated Selection

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