MAD1 (phospho Ser428) rabbit pAb

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],

Product Specifications

Background

UniProt

Q9Y6D9

Swiss Prot

Q9Y6D9

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human MAD1 around the phosphorylation site of Ser428. AA range:394-443

Clonality

Polyclonal

Source

Rabbit

Applications

IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Storage Conditions

-20°C/1 year

Fragment

IgG

Subcellular Location

Nucleus . Chromosome, centromere, kinetochore . Nucleus envelope . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . Co-localizes with TPR at the nucleus envelope during interphase and throughout the cell cycle (PubMed:22351768, PubMed:18981471) . From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody (PubMed:9546394) . Localizes to kinetochores during prometaphase (PubMed:22351768, PubMed:29162720) . Does not localize to kinetochores during metaphase (PubMed:29162720) . Colocalizes with NEK2 at the kinetochore (PubMed:14978040) . Colocalizes with IK at spindle poles during metaphase and ana

Other Product Names

MAD1L1; MAD1; TXBP181; Mitotic spindle assembly checkpoint protein MAD1; Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1; Mitotic checkpoint MAD1 protein homolog; HsMAD1; hMAD1; Tax-binding protein 181