AIP4 (phospho Tyr420) rabbit pAb

Itchy E3 ubiquitin protein ligase (ITCH) Homo sapiens This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012],

Product Specifications

Background

UniProt

Q96J02

Swiss Prot

Q96J02

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human ITCH around the phosphorylation site of Tyr420. AA range:386-435

Clonality

Polyclonal

Source

Rabbit

Applications

IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Storage Conditions

-20°C/1 year

Fragment

IgG

Subcellular Location

Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm . Nucleus . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . May be recruited to exosomes by NDFIP1 (PubMed:18819914) . Localizes to plasma membrane upon CXCL12 stimulation where it co-localizes with CXCL4 (PubMed:14602072) . Localization to early endosomes is increased upon CXCL12 stimulation where it co-localizes with DTX3L and CXCL4 (PubMed:24790097) . .