Ezrin (phospho Tyr478) rabbit pAb

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

P15311

Swiss Prot

P15311

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Ezrin around the phosphorylation site of Tyr478. AA range:446-495

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

70kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

70kD

Fragment

IgG

Subcellular Location

Apical cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection . Cell projection, microvillus membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cell cortex . Cytoplasm, cytoskeleton . Cell projection, microvillus . Localization to the apical membrane of parietal cells depends on the interaction with PALS1. Localizes to cell extensions and peripheral processes of astrocytes (By similarity) . Microvillar peripheral membrane protein (cytoplasmic side) . .