BUB1 rabbit pAb

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],

Product Specifications

Background

Product Name Alternative

BUB1; BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A

UniProt

O43683

Swiss Prot

O43683

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human BUB1. AA range:781-830

Target

BUB1

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

120kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

120kD

Fragment

IgG

Subcellular Location

Nucleus. Chromosome, centromere, kinetochore. Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, KNL1 and INCENP are required for kinetochore localization (By similarity) . .