Tak1 (phospho Thr187) rabbit pAb

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

O43318

Swiss Prot

O43318

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human MAP3K7 around the phosphorylation site of Thr187. AA range:161-210

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Molecular Weight

77kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

77kD

Fragment

IgG

Subcellular Location

Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Although the majority of MAP3K7/TAK1 is found in the cytosol, when complexed with TAB1/MAP3K7IP1 and TAB2/MAP3K7IP2, it is also localized at the cell membrane.