Krs-1/2 (phospho Thr183) rabbit pAb

Serine/threonine kinase 3 (STK3) Homo sapiens This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],

Product Specifications

Background

Product Name Alternative

STK3; KRS1; MST2; Serine/threonine-protein kinase 3; Mammalian STE20-like protein kinase 2; MST-2; STE20-like kinase MST2; Serine/threonine-protein kinase Krs-1; STK4; KRS2; MST1; Serine/threonine-protein kinase 4; Mammalian STE20-like prot

UniProt

Q13188/Q13043

Swiss Prot

Q13188/Q13043

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human Mst1/2 around the phosphorylation site of Thr183. AA range:149-198

Target

Krs-1/2

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

60kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

60kD

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . The caspase-cleaved form cycles between nucleus and cytoplasm (PubMed:19525978, PubMed:11278283) . Phosphorylation at Thr-117 leads to inhibition of nuclear translocation (PubMed:19525978) . .