Shc (phospho Ser36) rabbit pAb

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011],

Product Specifications

Background

UniProt

P29353

Swiss Prot

P29353

Reactivity

Human; Mouse

Immunogen

Synthesized phospho-peptide around the phosphorylation site of human Shc (phospho Ser36)

Clonality

Polyclonal

Source

Rabbit

Applications

IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Storage Conditions

-20°C/1 year

Fragment

IgG

Subcellular Location

Cytoplasm.; [Isoform p46Shc]: Mitochondrion matrix . Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.; [Isoform p66Shc]: Mitochondrion . In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation. .

Other Product Names

SHC1; SHC; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1