BLM (phospho Thr99) rabbit pAb

The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008],

Product Specifications

Background

Product Name Alternative

BLM; RECQ2; RECQL3; Bloom syndrome protein; DNA helicase; RecQ-like type 2; RecQ2; RecQ protein-like 3

UniProt

P54132

Swiss Prot

P54132

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human Bloom Syndrome around the phosphorylation site of Thr99. AA range:65-114

Target

BLM

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

159kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

159kD

Fragment

IgG

Subcellular Location

Nucleus . Together with SPIDR, is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR-dependent manner.