RPA32 (phospho Thr21) rabbit pAb
Function: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. PTM: Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2. subcellular location: Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. subunit: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.
Product Specifications
Background
Synonyms
Gene ID
6118
UniProt
P15927
Cellular Locus
Nucleus. Nucleus, PML body. Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner..
Host
Rabbit
Species Reactivity
Human,Mouse
Reactivity
Human; Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human RFA2 around the phosphorylation site of Thr21. AA range:10-59
Clonality
Polyclonal
Isotype
IgG
Source
Rabbit
Applications
WB, IHC, IF, ELISA
Validated Applications
WB,IHC,IF,ELISA
Stability
-20°C/1 year
Concentration
1 mg/mL
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Molecular Weight
35kD
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES7061-1.pdf
Observed Molecular Weight
35 kD
Subcellular Location
Nucleus . Nucleus, PML body . Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. .
Other Product Names
Gene ID (Human)
6118
SwissProt (Human)
P15927
Available Sizes
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