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CtIP rabbit pAb

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008],

Product Specifications

Background

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Synonyms

RBBP8; CTIP; DNA endonuclease RBBP8; CtBP-interacting protein; CtIP; Retinoblastoma-binding protein 8; RBBP-8; Retinoblastoma-interacting protein and myosin-like; RIM; Sporulation in the absence of SPO11 protein 2 homolog; SAE2

Gene ID

5932

UniProt

Q99708

Cellular Locus

Nucleus. Chromosome. Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, PubMed:25349192). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604)..

Host

Rabbit

Species Reactivity

Human,Mouse

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human CTIP. AA range:293-342

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

IHC, IF, WB, ELISA

Validated Applications

IHC,IF,WB,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Molecular Weight

100kD

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES7010-1.pdf

Observed Molecular Weight

100 kD

Subcellular Location

Nucleus . Chromosome . Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, PubMed:25349192) . Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604) . .

Other Product Names

RBBP8; CTIP; DNA endonuclease RBBP8; CtBP-interacting protein; CtIP; Retinoblastoma-binding protein 8; RBBP-8; Retinoblastoma-interacting protein and myosin-like; RIM; Sporulation in the absence of SPO11 protein 2 homolog; SAE2

Gene ID (Human)

5932

SwissProt (Human)

Q99708

Available Sizes

Curated Selection

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