RARα (phospho Ser77) rabbit pAb

This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus. [provided by RefSeq, Sep 2010],

Product Specifications

Background

UniProt

P10276

Swiss Prot

P10276

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Retinoic Acid Receptor alpha around the phosphorylation site of Ser77. AA range:46-95

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

45kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

45kD

Fragment

IgG

Subcellular Location

Nucleus . Cytoplasm . Nuclear localization depends on ligand binding, phosphorylation and sumoylation (PubMed:19850744) . Translocation to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation (By similarity) . Increased nuclear localization upon pulsatile shear stress (PubMed:28167758) . .