PAK5/6 (phospho Ser602/S560) rabbit pAb

The protein encoded by this gene is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008],

Product Specifications

Background

Product Name Alternative

PAK7; KIAA1264; PAK5; Serine/threonine-protein kinase PAK 7; p21-activated kinase 5; PAK-5; p21-activated kinase 7; PAK-7; PAK6; PAK5; Serine/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6; PAK-6

UniProt

Q9P286/Q9NQU5

Swiss Prot

Q9P286/Q9NQU5

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human PAK5/6 around the phosphorylation site of Ser602/Ser560. AA range:566-615

Target

PAK5/6

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

75kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

75kD

Fragment

IgG

Subcellular Location

Mitochondrion. Cytoplasm. Nucleus. Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival.