SphK2 (phospho Thr614) rabbit pAb

This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011],

Product Specifications

Background

UniProt

Q9NRA0

Swiss Prot

Q9NRA0

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human SPHK2 around the phosphorylation site of Thr614. AA range:580-629

Clonality

Polyclonal

Source

Rabbit

Applications

IHC; IF; WB; ELISA

Concentration

1 mg/ml

Dilution

WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Storage Conditions

-20°C/1 year

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Endoplasmic reticulum . Mitochondrion inner membrane . In nucleus, located in nucleosomes where it associates with core histone proteins such as histone 3 (PubMed:19729656) . In brains of patients with Alzheimer's disease, may be preferentially localized in the nucleus. Cytosolic expression decrease correlates with the density of amyloid deposits (PubMed:29615132) . In apoptotic cells, colocalizes with CASP1 in cell membrane where is cleaved and released from cells in an active form (PubMed:20197547) . .; [Isoform 2]: Lysosome membrane .