RFWD3 rabbit pAb

PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. similarity: Contains 1 RING-type zinc finger. similarity: Contains 3 WD repeats.

Product Specifications

Background

PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Contains 1 RING-type zinc finger., similarity:Contains 3 WD repeats.

UniProt

Q6PCD5

Swiss Prot

Q6PCD5

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Molecular Weight

85kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

85kD

Fragment

IgG

Subcellular Location

Nucleus . Nucleus, PML body . Cytoplasm . In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies (PubMed:21558276) . In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair (PubMed:21504906, PubMed:28575657) . This recruitment depends upon RPA2 (PubMed:21504906) . .