PP2A-Cα (phospho Tyr307) rabbit pAb

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

P67775

Swiss Prot

P67775

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307. AA range:260-309

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IP; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

35kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

35kD

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres (PubMed:16541025) . During mitosis, also found at spindle poles (PubMed:16541025) . Centromeric localization requires the presence of SGO2 (By similarity) . .